Re: [DIYbio] Re: What analyses should I have performed on this tissue?

On Sun, Dec 14, 2014 at 4:20 PM, Josiah Zayner <josiah.zayner@gmail.com> wrote:
> One would assume that with any tissue harvesting the sample would either be
> fixed or cryopreserved meaning the RNA should still be just fine.
>
I'm no expert, but from the extractions I have done of mammalian RNA
from FFPE slices or tissue stored in RNAlater, you will run into
degraded RNA. Degradation is usually caused by RNases, especially if
the samples are not preserved as soon as possible. Since RNases are
some common and hard to get rid of, proper preservation techniques are
the key.

That said, degradation may not have much affect on sequencing. My
colleagues have sequenced with degraded RNA but I think intact RNA
(RNA Integrity Number > 8) is preferred. I will have to talk to them
to find out.

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