On Mon, Dec 8, 2014 at 10:21 AM, Meredith L. Patterson <clonearmy@gmail.com> wrote:
I'd kinda put this on the "yeah, it'll happen someday" list but it's increasingly looking like "someday" is now. Any brain functional genomics folks have advice on where to start while I refresh myself on my notes from three years ago?
Just off the top of my head, and not a thorough list:
* Whole genome sequencing, and other variations of sequencing
* mRNA/transcripts, although the number of required samplings would make me think this could only get done as a DIY project (I don't see anyone doing neural tissue transcriptomics on scienceexchange)
* High-resolution scans, http://3scan.com/
* One day in the near future: synapse-resolution scans, http://brainbackups.com/
* Generally, there are many antibody-based tagging/visualization systems for fixed tissue. You'll have to browse through some antibody catalogs to figure out which particular methods would be most interesting to you. There are many antibodies or aptamers that can be made to specifically extract certain details from these samples.
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