In my experience, visible lux takes some time to produce. Perhaps just bioluminesence would be better, heat activation brings in a lot of complications
On Thursday, January 22, 2015 at 2:46:49 AM UTC-8, Lukas wrote:
-- Also, it takes quite a while to create yogurt strains (that I've worked with). I worked with one strain, S thermophilus, and it was a bit finicky with growth. Still, there's nothing stopping you from doing it.
If you have a bit bigger budget, perhaps pay someone to do the cloning of the lux operon for you. I have one lux (not tested with visible light production) cassette for Bacillus if needed, it's quite similar to S thermophilus and might be able to work. You'll be able to work quicker but perhaps not learn as much about cloning if you just get a company to clone the DNA, you can just work directly with yogurt. You also wont get very much variation, so troubleshooting would be more difficult
Where are you located? Perhaps a local biohacker lab can help you out with you idea
-Koeng
On Thursday, January 22, 2015 at 2:46:49 AM UTC-8, Lukas wrote:
Hey Guys
I want to create a thermosensitive yoghurt which reacts with bioluminescence under heat. I have the model of Meredith Patterson who has created a test for Melamin. I want to create the pudding for a school Project.
Lg Lukas
BTW if somebody has time : can you list the equipment for the experiment?
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