Re: [DIYbio] DNA extraction and DNA Barconding

On 01/15/2015 10:34 AM, Otto Heringer wrote:
> This way just a PCR and gel would be needed - and not sequencing for every barcode sample.
> I know some of you have some experience on the subject. What do you think about it?

No direct exp. yet, but sounds just like using a gas or liquid chromatograph output
to infer molecules in the sample. A PCR/electrophoresis only method would depend on molecular weight
and shape alone.

Would that give as much ability to discern as sequencing even a small part of a genome?
PCR/electrophoresis only might be like comparing search stats for
crime suspects by a height and body weight vs. right thumb print and shoe size.
The thumb print alone would trump
thousands of other matches based on height and body weight.

OK. Now the experienced can chime in...

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