Re: [DIYbio] DNA extraction and DNA Barconding

On Thu, Jan 15, 2015 at 11:34 AM, Otto Heringer <ottowheringer@gmail.com> wrote:

> I'll look for genomic DNA instead of only plasmids (I'm using this
> protocol), but the problem of where to find some reagents persists. The only
> one that is hard to obtain is indeed Tris.
>
Plasmid extraction is only for bacteria. It's more difficult to do,
because we want to keep the genomic DNA from contaminating the plasmid
DNA. If you got the right reagents, its not difficult. Since you are
looking to determine beef, then you're looking for genomic DNA.

>
> And about barcoding, I see that there is a still open debate about the use
> of barcodings because of the supposition that it might "replace" taxonomy.
> Some say it is only useful for "biodetection" of some species and that
> taxonomy need more complex work.
>
You can barcode using PCR/electrophoresis if you got the correct
primer designed. Is there a sequence found in cows that is different
from one in goats, chicken, etc? With that specific sequence, you will
have an expected PCR product size. Maybe you should find more than one
highly species specific sequence. Then you can have a "fingerprint".

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