Re: [DIYbio] DNA extraction and DNA Barconding

@Dakota

I'll play with the sequenced publicly avaliable genomes and see the mess that happens. I'm expetcing to get the obvious answer - "we use barcoding as we use because it is easier" - but also to learn something in the process.

@Matt and @Cory

Thanks for the recomendations!

@Manu

This is what I was expecting to someone shows up here! I should have searched for papers with this subject with another criteria. I would probably found this important article for what I want to do.

Thanks a lot for sharing this! It solves almost all the problems that I was expecting. I just need to search the same approach to identify earthworms - there's a urban legend that some fast foods use worms in their weird mix that is called "meat", haha

@Pen

Yes, but it will be needed to use restriction enzymes and if I had to choose between a restriction enzyme reaction on a whole genome and a PCR of specific regions, I choose the PCR. It is less messy to analyze the gel results.

@Sebastian

You are right. After Matt showed the companies selling those things to ppl like us (those weird creatures! #kidding) you got a better point than mine.

Thanks for the tip on the mitochondrial DNA kit. I'll take a look.

Also, I was thinking ask some chemists friends that were studing the protease activity of some tropical food (coconut, pineapple and papaya (papain!)) to give some information on the extraction viability of its proteases - I bet it is inviable, but I need to stop to speculate and search for the stuff even before asking them. It would be cooler get the proteases from the source instead from a industrialized product. :D

@Jeswin

It will probably be from the muscle (I hope so!). The sample is a crude Sfiha (I'll ask to be crude, it is not usually sold this way), an "arab mini-pizza" commonly found in some fast food companies menus here in Brazil.

Is a commom joke between my friends question the origin of the meat sfihas while eating it - something like: "Humm... *chewing*... They have put less paperboard on it this time." "Sure, this earthworm meat is fantastic today.".

2015-01-16 19:31 GMT-02:00 Jeswin <phillyj101@gmail.com>:

On Fri, Jan 16, 2015 at 11:05 AM,  <scocioba@gmail.com> wrote:
> well. I did a science project when I was a wee lad and extracted genomic DNA
> from chicken liver using household products. It worked but never did any pcr
> on it since this was in the 1990's and PCR machines were not easily found.
>


I also think you should get the DNA from tissues like liver. I think
other organs might be too tough. Not sure though.

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