Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII

3:32 AM |

Hi Sunil,

sorry to be the fun killer, but you should forget about home producing clinical-grade protein therapeutics. Slight contaminations or aggregation of your protein might lead to immunogenic reactions towards your therapeutic. And a second administration of your protein, after the patient developed anti-therapeutic antibodies, might be lethal in the worst case.

In our lab we also work on mice and drug administration of protein therapeutics. Hence I know about the high standards that need to be fulfilled to even keep your mouse alive.. ^^

Those things aside. Unless you are a really rich person, the costs of the equipment that is required for the expression, purification, packaging and storage steps is incredible. You would need a professional clean room to prepare your samples, pharmaceutical grade chromatography equipment, viral filtration, just to name a few...

I like your ambition as a DIY biologist, but the preparation of clinical/pharmaceutical therapeutics is far beyond the scope of a DIY project.

Cheers
Filip

2015-01-23 1:42 GMT+01:00 Sunil Phani <consultsunilphani@gmail.com>:
For bacteria 

On Fri, Jan 23, 2015 at 3:34 AM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
You mean a plasmid? For bacteria or plant?

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