ho sure pls send me , soo nice of you
i really appreciate your help ind this regard
and thanks for providing that recourse too..
but first get well versed with pVIB
With advance thanks
Sunil Phani
my address :
Sunil Phani K
Qtr No: B-8,
BDL Township,
Bharat Dynamics Limited,
Bhanur,
Patancheru Mandal,
Medak Dist.
Hyderabad-502305,
Telangana.
Mobile:+91-9490123821.
On Sat, Jan 24, 2015 at 7:48 PM, Mega [Andreas Stuermer] <masterstorm123@gmail.com> wrote:
I only have pVIB. If you want I can send you.--Or you order the GFP plasmid from http://www.carolina.com/biotechnology-plasmid-lambda-dna/pgreen-plasmid-1-ug-200-ul-0005-ugul/211449.pr?catId=&mCat=&sCat=&ssCat=&question=plasmidWith it the colonies are even green under normal light
On Saturday, January 17, 2015 at 3:01:14 AM UTC+1, Sunil Phani wrote:can someone please help me setup DIY-BIO home Lab to perform mammalian cell culture to produce Recombinant Factor VIII protein with mammalian cells(CHO, HKE 293, HEL, etc) and kindly provide me recombinant dna protocols for identification, isolation , extraction, insertion and expansion .my requirement is to prepare pilot project in small scale DIY-BIO lab which can further be scaled.
if possible provide me vectors, cell line and seedstock. i am new to DIY-BIO hence i need helping hands of veterans like you ppl .. your help be highly appreciated
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