I found a link for a previous researcher who made a DIY set-up for observing GFP: http://wormlab.rice.edu/LED/
-- Do you think that if I replaced the filter for GFP observation with one for YFP observation, it would work?
On Saturday, February 14, 2015 at 5:23:47 PM UTC-8, paul wright wrote:
On Saturday, February 14, 2015 at 5:23:47 PM UTC-8, paul wright wrote:
Hi,I am doing a project on the effect of certain compounds on the accumulation of a protein, aSynuclein, in C. elegans. (aSynuclein being a common indicator of Parkinson's disease)The C. elegans strains I am using are OW13 and NL5901. In both strains, aSynuclein in the body walls of the worms is marked by Yellow Fluorescent Protein.I currently do not know how to image the worms. (I assume that I need to shine UV light on the worms in a dark setting to see the glowing, but I am not sure)I do not have access to an epifluorescent microscope, but I do have access to a dissecting microscope and UV light.Any input on how to take pictures of the fluorescence of the YFP would be appreciated!
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