Emailing with Iliana, but I will also post here so others can find the information if they want to.
Iliana,
How many sequence types do you have that are common to all species?
You will have to align them. You can do this either individually by sequence type, or make a concatamer. I'd suggest you do both and see what the results look like. (Note: don't just dump all the sequences and align together.)
You can use this NCBI page to make the alignments:
Note that the box "align two or more sequences" must be checked.
Afterwards you can make the tree with your alignments using a variety of different types of software, for example:
Try different options and see what they look like.
Note: missing data for a species for a sequence type means you can't use that species in either a single-sequence tree or a concatamer tree. Don't just leave a blank in the concatamer :)
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/304fc58b-daac-4cae-bbb6-78675ec567b1%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment