guys sorry for delay in my postings while i was
studying a lot about adeno associated viruses and retroviruses and retroviruses, exploring several possibilities guys pls don't be so conventional and conservative
ofcourse they are safe ... just think of ex-vivo
why is world of biotech not moving for the past 30 years...
while other branches of sciences like Computers and IT emerged in a strong way in 30 years
we are failed to produce non of the path breaking new ideas at all..
may be too much conservative thinking emerged now a days
or may be too much churches influence
or may non presence of openness and democracy in biotech
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
can someone please help me setup DIY-BIO home Lab to perform mammalian cell culture to produce Recombinant Factor VIII protein with mammalian cells(CHO, HKE 293, HEL, etc) and kindly provide me recombinant dna protocols for identification, isolation , extraction, insertion and expansion .my requirement is to prepare pilot project in small scale DIY-BIO lab which can further be scaled.
if possible provide me vectors, cell line and seedstock. i am new to DIY-BIO hence i need helping hands of veterans like you ppl .. your help be highly appreciated
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