[DIYbio] Re: Plasmids as a vector

The other replies probably give a lot fo info for you already, but here are my two cents:

1 How can I bring the Vector that is in the cell plasma because of de
Electroporation into the Genome of the Cell?

Are you talking about the E. coli bacterium? If so, then you don't need to integrate the plasmid into the bacterial genome. The genes you cloned in will express fine from a separate plasmid after electroporation. This is the standard procedure to express foreign genes in E. coli. Although there is technology available to integrate plasmids into the E. coli genome (as someone mentioned above), you don't need it. If just prepare a plasmid with the right gene on it and put it into the bacteria, then it will express fine as it is.

 
2 I have seen the website http://www.addgene.org/ there it gives many
plasmid. Are this also vectors? And in there Plasmid it has Promoters,
Parts for restriction enzyme and ORFs. But is in the ORFs already some
codes for Proteins or must I add this? And can I find there a vector for
plants?

The first question answered was perfectly answered above.
For the second, if you look at an expression plasmid, then it should have the proper expression cassette (promoter - multiple cloning site - end of gene) to work. If it does, then you need to cut the plasmid at the multiple cloning site, insert your protein code of interest, and your expression plasmid is then ready.
Transfecting plants is a lot harder than transfecting bacteria. Some plants can be transfected via the agrobacterium system, others using plant viruses, and for most plants, we don't really know yet how to transfect them. Either way, its a lot more challenging than E. coli. So I wouldn't attempt transfecting plants without a solid knowledge of cloning in E. coli.
Also, don't forget that cloning is under strict regulation in most places, so unless you have the proper clearances, you cannot perform such experiments.

On Saturday, 7 February 2015 22:08:55 UTC, Louis Weber wrote:

Hey Guys

First of all sorry for my bad English. I`m a beginner and need some help about plasmid.

So you can use plasmids for cloning (to copy a DNA-Part many time in a Bacteria), but also as a vector for gene transfection or transformation is that right?

So for Example, can I design a Vector and put it with for example with electroporation in the cell, and the Cell would, if I had de right Promoter, transcript the DNA and produce the Protein that I had on the Vector?

If that's right, I had two questions…

1 How can I bring the Vector that is in the cell plasma because of de Electroporation into the Genome of the Cell?

2 I have seen the website http://www.addgene.org/ there it gives many plasmid. Are this also vectors? And in there Plasmid it has Promoters, Parts for restriction enzyme and ORFs. But is in the ORFs already some codes for Proteins or must I add this? And can I find there a vector for plants?

Sorry for my  questions but I am a beginner and found not very much information on the internet.

Louis