Check out the input and output wavelengths for a few fluorescent proteins (including YFP):
This photo/video industry filter sample pack is $2 and is probably a good place to start (an iGEM project recommended trying these before settling on more expensive filters)
On Sat, Feb 14, 2015 at 5:23 PM, paul wright <paulwrightpaulwright77@gmail.com> wrote:
Hi,--I am doing a project on the effect of certain compounds on the accumulation of a protein, aSynuclein, in C. elegans. (aSynuclein being a common indicator of Parkinson's disease)The C. elegans strains I am using are OW13 and NL5901. In both strains, aSynuclein in the body walls of the worms is marked by Yellow Fluorescent Protein.I currently do not know how to image the worms. (I assume that I need to shine UV light on the worms in a dark setting to see the glowing, but I am not sure)I do not have access to an epifluorescent microscope, but I do have access to a dissecting microscope and UV light.Any input on how to take pictures of the fluorescence of the YFP would be appreciated!
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-Nathan
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