Re: [DIYbio] gel electrophoresis vs liquid chromatography?

On 02/18/2015 01:57 PM, GO wrote:
> I would love to simplify detection (at least) to have minimum of consumables possible. Any ideas?

Perhaps electrophoresis is just a hangover from what was simple and possible.

The gap now is several things:
1. That pressure plumbing requires skill, or special engineered precise fittings for connections
to columns, and the pipping is skinny tubes that can become dirty and be a difficult
cleaning/decontamination job, or an expensive replacement job.
2. That spectrometers or monochrome detectors need precise time stamping to be able
to use the resulting chromatography delay. That means a dedicated computer system...
3. Costs.

It's high time to reduce the costs and the controllers to handle the precise data logging are here too.

For controlling this kind of thing, I like micropython with an ethernet port, but a Black Swift linux board
with wifi instead could be good. Both also have USB, but ethernet and a web server could be nice.

So, if you used a black swift, the linux computer with wifi part is just $25. Add sensors, IO, relay drivers
and secured code-->viable product affordable to DIY/low budget folk.

It also could be a reason to have 6 LC machine setups in a lab instead of just one. Each could have
a certain type of column in it, reducing contamination events since it stays
assembled waiting for use all the time.

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