So if the strains are less of an issue than regeneration, what pushes you to use GV3101 over another strain? Or more generally speaking, what would be the factors to consider when pairing strains to species for this process?
Thanks for the tips on regeneration, got me going in a good direction. I also appreciate the density of your grammar.... helps me learn more, faster
On Sunday, February 15, 2015 at 4:17:33 PM UTC-8, Sebastian wrote:
-- Thanks for the tips on regeneration, got me going in a good direction. I also appreciate the density of your grammar.... helps me learn more, faster
On Sunday, February 15, 2015 at 4:17:33 PM UTC-8, Sebastian wrote:
I use GV3101. Other popular strain is EHA105. Swtchgrass, being monocot, is harder to regenerate and agro isn't hugry for it since it lacks large phenolic efflux during wounding which induces virulence in agro. Hardest part isn't strain but a reliable regeneration protocol. Leaves of grasses are tricky to induce somatic embryogenesis. Pardon grammar and brevity, on mobile but wanted to chime in.
Silver nitrate and alpha phytosulphokine works in maize for induction. Pricey medium and light sensitive but can be done. Take protocols with grain of salt since most unless well cited are hard to reproduce. Some cultivars of commonly used crops are distributed for free or low fee from the USDA to business and institutions. Try to replicate experiments using same tissue source or email author for materials.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Nico B.
Sent: 2/15/2015 6:17 PM
To: diy...@googlegroups.com
Subject: [DIYbio] Picking the right strain of A. tumefasceins for 'genejockeying' P. virgatumWorking on a hobby project to make a transgenic switchgrass with an expression of CYP2E1 and working with snap gene as a complete novice. I've found over a dozen varieties of A. tumefasceins species that seem to be dependent on the targeted plant whether that may be a poplar hybrid, petunia, etc., not sure what the considerations are for each of those, though.--
How could I go about figuring out what strain is appropriate for my desired function per plant?
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