Off the top of my head, I don't know of any integrative plasmids for E. Coli. But a quick google had this on the top, http://www.jbioleng.org/content/7/1/12 which explored chromosomal integration of BioBrick parts. In general, most of the mechanisns seem to revolve around techniques that viruses have used to "take over" a host cell.
I've switched to focusing mainly on Streptomyces and Actinomyces now, and there is a phic31 integrase vector that exploits a viral mechanism for integration of your plasmid DNA Into the chromosome of the bacteria.
I am guessing there are also integrative plasmids in E. Coli, but I don't know what size limitations they have, nor do I know the names of the plasmids. Some literature searches should probably give you an answer though.
Do you have a specific protein in mind?
On Sun, Feb 15, 2015 at 2:50 PM, Jeswin <phillyj101@gmail.com> wrote:
I don't know too much about this, but I think you should be looking
into expression vectors. I'm not sure how you would incorporate the
plasmid DNA into the genome, however, you can use plasmid expression
vectors to produce proteins of interest.
--
On Sat, Feb 7, 2015 at 5:08 PM, Louis Weber <louis.weber1983@gmail.com> wrote:
> Hey Guys
>
> First of all sorry for my bad English. I`m a beginner and need some help
> about plasmid.
>
> So you can use plasmids for cloning (to copy a DNA-Part many time in a
> Bacteria), but also as a vector for gene transfection or transformation is
> that right?
>
> So for Example, can I design a Vector and put it with for example with
> electroporation in the cell, and the Cell would, if I had de right Promoter,
> transcript the DNA and produce the Protein that I had on the Vector?
>
> If that's right, I had two questions…
>
> 1 How can I bring the Vector that is in the cell plasma because of de
> Electroporation into the Genome of the Cell?
>
> 2 I have seen the website http://www.addgene.org/ there it gives many
> plasmid. Are this also vectors? And in there Plasmid it has Promoters, Parts
> for restriction enzyme and ORFs. But is in the ORFs already some codes for
> Proteins or must I add this? And can I find there a vector for plants?
>
> Sorry for my questions but I am a beginner and found not very much
> information on the internet.
>
> Louis
>
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