Re: [DIYbio] Plasmids as a vector

On Sun, Feb 15, 2015 at 2:54 PM, Dakota Hamill <dkotes@gmail.com> wrote:
> Off the top of my head, I don't know of any integrative plasmids for E.
> Coli. But a quick google had this on the top,
> http://www.jbioleng.org/content/7/1/12 which explored chromosomal
> integration of BioBrick parts. In general, most of the mechanisns seem to

These allow DNA to be added to the genome? I had wondered if Louis was
talking about this, but I think he is looking for a protein expression
vector. Yeah...not knowing terminology can really hurt. At one point
in my lab experience, I thought heat blocks needed water in them, so I
referred to them as water blocks...so colleagues gave me that funny
look until I described it.

--
In necessariis unitas, in dubiis libertas, in omnibus caritas.
-Marco Antonio Dominis

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