The only time I've ever seen an HPLC used for DNA is purification of short oligonucleotides like primers for PCR. Awesomely enough (is that a word? No, but I'll use it.) it was a Waters 600E which looks like its from the 60's and was part of NASA's command launch console for the first moon missions. In fact, it was so old the UV detector simply read as a needle that went up and down, not even an LED display. The tech would stand there and collect the fraction by hand until the needle dropped down, then stop.
I know that company in Canada that does magnetic bead based DNA assembly uses it for purification of their specific oligos. Genomikon I think they are called.
I've never heard of large pieces of DNA being purified by HPLC, I imagine the combination of the pressure and column/silica might shear it? Depends on the stationary phase though I suppose, but I've never heard of anything besides oligos being done by HPLC. A little bit of refined sea-weed and a DNA stain and you're good to go in 20 minutes.
There are capillary electrophoresis machines which I think can separate DNA, and perhaps even fraction collect, but I've never been around them on a regular basis.
On Wed, Feb 18, 2015 at 8:59 PM, 'SC' via DIYbio <diybio@googlegroups.com> wrote:
I've never collected a DNA band from HPLC. How is it done?--
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