Re: [DIYbio] Re: gel electrophoresis vs liquid chromatography?

Are you talking about a capillary filled with agarose as a means of
eluting DNA products? The closest thing I know where you can collect
your DNA of interest without cutting is using the Invitrogen e-gel
cassettes. It's a pre-made gel formed in a plastic square with
electrodes at each end. You place this on a special instrument that
applies voltage. The cassette is not immersed in any running buffer
and at the middle of it is open lanes which you fill with a elution
liquid. When your sample reaches the collection lane, simply pipette
out the liquid. It's pretty neat, but quiet expensive compared to
regular agarose gel electrophoresis.

On Thu, Feb 19, 2015 at 10:32 AM, Dakota Hamill <dko...@gmail.com> wrote:
> You're on the right track of thinking though.  I don't know how well the
> Pippin Prep works, I've heard mixed reviews.  There is always room for
> improvement.  There was a lab someone showed me at Boston University that
> made a pretty cheap (I think) fully functional "PCR machine on a chip" the
> size of a glass slide.  I think it works by cycling fluid over pre-heated
> areas that denature, anneal, elongate, etc.  Being able to do that, then 30
> cycles later send it out another channel directly into a "gel on a chip" or
> something else would be cool.
>
> On Thu, Feb 19, 2015 at 10:25 AM, Dakota Hamill <dko...@gmail.com> wrote:
>>
>> You don't need to elute it off the gel, you can just excise it with a
>> razor blade and prep your DNA from it.  That's a common practice.
>>
>> As for your wells on a gel idea, a local company here has done that.  Sage
>> science with the Pippin Prep.
>>
>> On Feb 19, 2015 10:23 AM, "GO" <gobi...@gmail.com> wrote:
>>>>
>>>> Thanks you all, people! It certainly helps to discuss this. By the way,
>>>> sorry for the bad grammar, I was in a hurry.
>>>
>>> I agree that huge pressures in HPLC is a drawback. I totally missed but
>>> obviously one can use the gel several times and even elute at the end so
>>> basically with few modifications it is the same system. For example, if one
>>> could put a detector at the end of the gel and make collection wells at the
>>> end, it would be the same just simpler because voltage would drive it
>>> instead of pressure. And we wouldn't need labeling if detector is for 260nm.
>>>
>>> >Stacy: I agree, HPLC is prone to more problems. Collection in HPLC is
>>> > very easy, just need to put your tube at the end of the tubing (any point
>>> > after the column and detector) and have some time estimate of distance
>>> > between the detector and the tubing end. The sample would be diluted though.
>>> >John: Pressure is a bummer but I don't agree for timing. The typical run
>>> > is not so fast and timing is not a problem IMO but as for electrophoresis
>>> > you would need dedicated system (like power supply). Just since you are
>>> > using eyes for electrophoresis, detection is easier.
>>> >Dakota: Good point about the size. I looked it up, and they can do
>>> > bigger dna but with the ion exchange column protocol so it is more
>>> > complicated.
>>> > Nathan: Unfortunately I don't have any good ideas right now but my goal
>>> > would be to make detection of biomolecules so easy that average Joe can do
>>> > with with one device at home and no consumables. I guess the kits are the
>>> > closest thing for that but the fact that you can't run the same thing
>>> > repeatedly is a major setback. You need to purchase kit or stick (for drug
>>> > or pregnancy detection) every time. Actually I need to take a look at those
>>> > diabetics sensors/dispensers.
>>> Regarding the agarose microchannels, I made some microstamps with agarose
>>> so my guess is that charge is not your problem but more likely that you
>>> didn't keep the whole thing hot when you tried to get agarose inside. If it
>>> is gelled than it breaks easily. I can send you the images that I took and
>>> we can discuss. I am interested in that project but did you search the
>>> papers (I am pretty sure it was done before).
>>>
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