I am copying my laboratory's parts collection for a collaborator. The library is naked DNA. I was going to use phusion PCR to amplify the parts and then just send him the dirty pcr products, but im wondering if I should deactivate the phusion polymerase at least. The only issue is that phusion doesnt denature with heat.. should I denature with something else, use a different polymerase, or not even worry about denaturing?
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