A mouse promoter should work without problem. For the yeast one, it is a good question. I don't know how efficient an endogenous yeast promoter is in mammalian cells. I suppose there should be some activity but I am not sure if it will be at the same level as the one you see in yeast.
-- Looking at this paper, a human promoter should be ok since the length of homology required for an efficient crossover is in kb whereas a promoter is below 500 bp.
http://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1018&context=rivera
On Monday, 30 March 2015 18:19:28 UTC+1, Mega [Andreas Stuermer] wrote:
On Monday, 30 March 2015 18:19:28 UTC+1, Mega [Andreas Stuermer] wrote:
How about using a mouse promoter or yeast promoter? It will be different...
On Friday, March 27, 2015 at 11:19:46 PM UTC+1, Nathan McCorkle wrote:I've got an idea that you could make a condom-catheter type
microfluidic. I've read about male/female sorting of sperm, but how do
you edit the DNA? I know about CRISPR, transposons, and probably some
other similar things I can't remember, but any other ways that have
been thought of or experimented with?
Is adding a new chromosome a better idea? Would this prevent the 'new'
chromosome from being passed during future breeding with a
non-modified person? (I think that would make the neo-human less
offensive to anti-GMO folks)
Previously I've asked what political/social climate would be most
conducive to this work, and I think the answer was 'south korea', but
that was a few years ago, and I wonder if there's a better place these
days. Not necessarily a country with active research, but one that
would be supportive of active evolution, rather than being fearful?
--
-Nathan
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