I think it's called sfGFP. That's the most effective one.
-- Or, you could do what I used to do, which is just keep it constantly expressed and grow the cells for much longer. Worked for me as well
I also have that plasmid that they use if you would like a sample. Ironically, it has the GFP gene regulated by the lac operator yet still expresses well under normal conditions. Probably because there are more plasmid copies than lac repressor proteins in the cell, but I've never really investigated it.
-Koeng
On Monday, March 16, 2015 at 12:50:13 PM UTC-7, Mega [Andreas Stuermer] wrote:
On Monday, March 16, 2015 at 12:50:13 PM UTC-7, Mega [Andreas Stuermer] wrote:
Hi guys!I was wondering if anyone knows where to get the sequence of the mutant GFP that makes E coli look green even under daylight...
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