According to wikipedia, one molecule was speculated to be able to kill a cell. However, pUC Ori copy number is 100 to 500 per cell and so you should have tons of colV to kill other cells.
I haven't looked into the immunity mechanism but it involves blocking the TolC receptor ehere colicinV usually binds too.
You could definitely try to induce colV-immunity with IPGT to see if that increases survival.
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Re: [DIYbio] Colicin V (or microcin V or ColV) is a poor solution for DIYbio transformations
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