Here's 2 articles that I looked into a few years back when my lab was dealing with contamination. As I recall, we had qPCR experiments with unexpected positives. I believe I also got positives in purification column controls. I forget how it got resolved but I'm not surprised you get contamination during the manufacturing process.
Evans, Gloria E., et al. "Contamination of Qiagen DNA extraction kits with Legionella DNA." Journal of clinical microbiology 41.7 (2003): 3452-3453.
Tilburg, Jeroen JHC, et al. "Contamination of commercial PCR master mix with DNA from Coxiella burnetii." Journal of clinical microbiology 48.12 (2010): 4634-4635.
On Mon, Mar 23, 2015 at 10:26 AM, Avery louie <inactive.e@gmail.com> wrote:
http://www.virology.ws/2013/09/18/de-discovering-pathogens-viral-contamination-strikes-again/
Tl,dr; silica columns contaminated with virus, causes false positive. Pretty interesting, thought you all would enjoy it if you have not seen it.
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAL4KOmjtrqQ_sEzeC3j%2BwP2Mq193D8kbQCy5sMX2FRufirRhkQ%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
--
In necessariis unitas, in dubiis libertas, in omnibus caritas.
-Marco Antonio Dominis
-Marco Antonio Dominis
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAAhF0RKvFdK2GOK3ZdnZY87F_Rq2uzb1wcatw6xy9bVfE1Xw0g%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment