Hi,
This is definitely true for all the cell line storages I have ever dealt with. The key is long-term storage. Human cells are okay at -80 for a month or two, but after a year of storage at -80 it is strongly advised to discard them. I was told that viability decreases, and even if the cells do thaw, they will contain epigenetic and other changes that will ultimately change the cell line and this is not what we want. I never tested this though, but I accepted it as this is the norm everywhere.
Liquid nitrogen (-200) is the recommended long term storage for human cells.
Mate
On Monday, 20 April 2015 18:01:47 UTC+1, Mega [Andreas Stuermer] wrote:
-- This is definitely true for all the cell line storages I have ever dealt with. The key is long-term storage. Human cells are okay at -80 for a month or two, but after a year of storage at -80 it is strongly advised to discard them. I was told that viability decreases, and even if the cells do thaw, they will contain epigenetic and other changes that will ultimately change the cell line and this is not what we want. I never tested this though, but I accepted it as this is the norm everywhere.
Liquid nitrogen (-200) is the recommended long term storage for human cells.
Mate
On Monday, 20 April 2015 18:01:47 UTC+1, Mega [Andreas Stuermer] wrote:
Hi everyone,
Today at University I heard that human cells from the -80°C freezer often don't work after thawing. Especially when being frozen for a year or more.
I always thought -80°C would work well and -130°C would be better but optional.
Is this due to our freezer, or do you have similar experience?
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