Thanks, I will try that ;)
On Monday, August 31, 2015 at 9:26:07 AM UTC-3, Mega [Andreas Stuermer] wrote:
-- On Monday, August 31, 2015 at 9:26:07 AM UTC-3, Mega [Andreas Stuermer] wrote:
http://www.researchgate.net/publication/224812866_ Development_of_versatile_ shuttle_vectors_for_ Deinococcus_grandis I googled for "D. Plasmid", often they characzterize minimal plasmids by cutting them and seeing what replicates in the species.
Worst case you can always try carbon whisker or electroporation
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