[DIYbio] Re: Multiplexed crispr genome editing

There's really no reason to use this in prokaryotes because you can use the native spacer repeat system and have that processed correctly. No ribozymes needed!

However, you do need to use it for cells with nuclei because the CRISPR repeats can't be processed correctly. The most important part is apparently the 3' end, which has to have a clean stop.

-Koeng

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