(meaning I got ~10x more colonies with 20bp vs 40bp)
On Tuesday, November 17, 2015 at 11:12:27 AM UTC-8, Nathan McCorkle wrote:
-- On Tuesday, November 17, 2015 at 11:12:27 AM UTC-8, Nathan McCorkle wrote:
On Mon, Nov 16, 2015 at 7:06 AM, Koeng <koen...@gmail.com> wrote:
> Recently I've begun using SLiCE for cloning and have got some of my labmates
> to jump aboard. From about 1-2 weeks of trials, here's what I've found with
> single part SLiCE assembly. In the future, I hope to try 1. PEG8000 to
> increase SLiCE efficiency and 2. Multipart assembly 3. using homemade comp
> cells.
>
> Important stuff: SLiCE is approx. 1/3 the efficiency of homemade gibson
> assembly. Gibson increases efficiency with additional homology while SLiCE
> decreases. The optimal homology length for gibson is ~40 while the optimal
> homology length for SLiCE is ~20. SLiCE
Does you last sentence mean SLiCE won't work with more homology? Have
you verified this?
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