Re: [DIYbio] Tillandsia usneoides tissue culture and transforamtion

Saying that culturing spanish moss, where tissue culture has more physiological rather than phylogenic factors to account for, like you would pineapple may not be the best advice. Thats like saying to care for a manatee one should look to literature on elephant husbandry since they are very closely related. Even different cultivars within the same species may have drastically different hormonal responses (annecdotal) so take any literature protocols you find with a grain of salt but do try them out. You never know...you might just get lucky! Although members of the "airplant" family (also bromeliads) may give you some decent leads on micropropagation, as Yuriy stated its a bit scarce post cursory google search especially now that sci-hub is down.

The thin long leaves will be tricky to surface sterilize and may damage those awesome dust-eating tissues on the surface. The high surface area due to said cells will require some dish soap along with bleach to break the tension to get all up in there. Bromeliad seeds suck at germinating so leaf tissue is your best bet...especially for the moss who lays vegetative for most its life.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC


> On Nov 11, 2015, at 11:22 PM, Yuriy <yuriyology@gmail.com> wrote:
>
> You people do know that the term Spanish "moss" is a misnomer. Right?
>
> As for the original poster. Lookup "pineapple transformations". It's the closest commercially viable species thus most researched. Still works with agro.
>
> Bromeliad transformations coughed up next to nothing in search.
>
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