Okay so at this stage we don't really have enough understanding of proteins to be able to look at a protein and create something, based on how the protein is structured, that would be guaranteed to effect it in a certain way. I'm guessing the computer models are as advanced as they can be given the existing combinations that have been tested - so just curious what is it that is holding them back? Is it just that proteins are so complex that we haven't viewed enough to put together a fully accurate set of rules (ways to resolve those 1-5)?
On Friday, December 11, 2015 at 6:06:41 AM UTC-5, DrBrian wrote:
-- For the phage - Sorry if this is redundant or really straightforward but I'm not sure I understand fully. So what you are saying is that if I figure out which protein to target and a specific receptor, then figure out what will bind that receptor, then I could attach the colicin domain to the binder and it would potentially enter and kill the bacteria? Also this would assume that the protein targeted is the one that was responsible for persister survival, right? Or it could really be any conserved protein? So this would go on the assumption that the mechanism for persister survival would not be triggered by this attack? I guess I'm trying to understand if this approach could be used to disable or circumvent the survival mechanism of the persisters. Or would I really need to look at the atomic level structure to figure out the exact persister mechanisms and how to disable them? So far we know how to kill the bacteria - to a point- and then the persisters survive all treatments tested.
That STM looks great! I'm not sure yet if I'l need to look at atoms to be able to accomplish this or not.
Thanks again sorry if I'm repeating things I'm just trying to understand everything.
David
On Friday, December 11, 2015 at 6:06:41 AM UTC-5, DrBrian wrote:
Such a great thread you have started David!If you are looking for more techniques a Scanning Tunneling Microscope was built at this years berlin science hack day.----------Documentation concerning the attempt to build an STM, a scanning tunneling microscope, at Fab Lab Berlin. With the microscope it is possible to see individual atoms and enter the world of quantum mechanics.https://wiki.fablab.berlin/STMOn Fri, Dec 11, 2015 at 8:47 AM, Mega [Andreas Stuermer] <masters...@gmail.com> wrote:You actually don't have to test protein-protein interaction in the early stage if a phage and its binding site has been characterized. Take the opportunity to stand on the shoulders of giants.
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