Re: Copper beads as thermocycling "block" - Was: [DIYbio] What do open source PCRs need?

On 02/11/2016 09:17 PM, Mac Cowell wrote:
> Consider a hot air convection thermocycler: is it better to preheat the airstream away from the sample and maximize air speed, or
> is the reduced effectiveness of the slower airspeed that results from packing the heater around the samples offset from conductive
> heat transfer?

max air speed will give max heat transfer. Stirring by going past turbulence generating fins is needed to avoid laminar flow
where different layers can have different temperatures. Heater around the samples will definitely make the air slower, and
temperatures less even. Beads of metal will just be intermediate heat capacity creating heat flow lag.

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