Re: [DIYbio] Re: PCR trouble

I'm going to try again with better template tonight and another parallel run with some DMSO added. If they don't work I'll do a digest tomorrow night and see what I get. I may also run a 16s PCR too just to check.

If the digest doesn't work right I'll get primers for another large amplicon from a different plasmid that's had fewer generations since it was made and better sequence data (pDusk probably) and run that to see if the trouble is drift in the target sequence.

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