On Saturday, March 5, 2016 at 8:44:54 AM UTC-8, William Beeson wrote:
Nice data point! They say "cells lost 94.5% of TE after only 24 hours of storage at -20°C. Cells lost 98.9% of TE after 2 days, and 99.6% of TE after one week of storage at -20°C" - so that would be 18x after one day, 91x after 2 days, and 250x after 7 days.
-- for their ultra-high competency cells they have a FAQ (https://www.neb.com/faqs/1/01/01/can-i-store-competent- ) that says after 1 week of storage at -20C the efficiency is decreased by 250x.cells-at-20-deg-c-instead-of- 80-deg-c
Anyone know whether electrocompetent cells maintain their competence any better, or whether there are any particular strains that tend to maintain competence longer when stored above -80?
Patrik
PS: Of course, all this also points out how important shipping conditions are likely to be for competent cells
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