I've built 3 flow hoods, and refined the design a little each time. My main desire was to have a large workspace, which I couldn't really get with most designs out there. I've had two large commercial units I bought at auction for next to nothing. But I never ended up using either of them. Replacing the filter would have been way too expensive, and I didn't like the open front designs of them. For me it is important to have a full window on the front. I open it, use it, then shut it afterward. The inside stays clean and I often leave cultures in there since it's a nice clean space.
-- I'll try to dig up some pics or take some new ones for you.
One thing I found was that blowers always vibrate, and if they're directly attached, or the hood is not fairly heavy, it vibrates your work surface a bit. This makes some things a lot harder than they should be.
The first super-cheap design I used was the same as this...
https://files.shroomery.org/files/04-06/621223049-glovebox5.jpg
I turned the filter unit upside down and glued or screwed it directly to the bottom of the bin. That avoids the goofy plastic duct on the back and makes a more solid unit. I also caulked the filter into the unit, including the edges of the filter. Then I taped over the seams and switches on the unit to make sure the body was airtight.
I don't remember doing any dish tests on it, but I don't recall having any problems with contamination using it.
On Tuesday, April 19, 2016 at 9:04:52 AM UTC-7, bostjan wrote:
On Tuesday, April 19, 2016 at 9:04:52 AM UTC-7, bostjan wrote:
Hey everyone, thanks for all the great feedback!William, I agree that if there are commercial household/hobby alternatives available, they will probably make a better/cheaper alternative than a DIY device and I invite you to add such items to the review if you find some. I will also try to add a few my self. Also considering safety, that is a good point! I think we could add that to the review, however, it will take some time =)Jake, thanks for the input on the laminar flow hoods/clean benches, I know what you mean...actually I was wondering whether it was worth it to make a DIY build that was nearer to the commercial devices. At the moment, I just work around a bunsen-burner when I have to transfect some samples in a "near sterile" environment, which is ok if it can be "quick and dirty", but it's not a long term solution if you have multiple samples...Ilya, I agree and you are very welcome to contribute to the Github reivew.
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