I also had some thoughts on a similar design, I don't see any reason why it shouldn't work, pcr technology actually started like this: 3 waterbaths and a lot of patience. Fluorescence in situ hybridization is often still done like this and similarly includes denaturation and annealing steps.
If you have more space available on the printing plate, you could increase the water surface by cutting the bottles along the other edge: this would allow to use less water and definitely more tubes!
On Thursday, 11 February 2016 20:57:03 UTC+1, VladimirGent wrote:
Hello , I am interested on community opinion on my new project for DIY-Bio. A PCR-mashine that can be created in any place. From scratch. 50$. No complex electronics.. very old technology.
It is on Kikstarter now, but I will finish the project anyway.
Educational project.. DIY-Bio, PCR-machine for amateur bio lab, .. universal design for experiments with PCR process.
.................story there.........PCR reaction is used in molecular biology to make copies of DNA-molecules, encoding our genes. To run the classic version of such reaction one need a PCR-machine, essentially thermocycler.
Existing DIY versions are too complex to make by amateur biologists.
So, I have made a special design of PCR mashine that is easy to reproduce everywhere in the world with minimal knowledge and skills in electronics. No Arduino , no thermostat modules, pure steam-punk version.. made from recycled parts. And very easy in case of repair and maintenance.
It is functional , and educational as well.
It can be made by school-children in science-class from the materials found in trash-can in the nearest FabLab, and Law of Om and a little bit of Thermodynamics..
It can be run full-manually , or using a 3D printer as automatic hand.
It involves the participants into the magic of science.
The result of that project is going to be a video with demonstration and instructions how to build up your own thing like that and how to use it ... And text also of course...It is Open Project... everyone can make own version based on the principles and using things that are readily available.
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