[DIYbio] Re: Which online store to procure yeast (for culturing) for small to medium quantities?

I'm currently working on a small DNA repository. It's taking a while because I'm optimizing all the protocols and plasmids to be extremely simple to use. I'm planning on packaging the DNA of the repository in phage, which costs nearly nothing to make and is stable for long periods at room temperature, which is nice for storage and large scale distribution. My current target organisms (ones which will have well characterized base vectors) are E coli, S cerevisiae, and A thaliana. It'll mainly be set up so people can contribute CDSs while I create/handle characterization and standardization of the basic plasmids. The idea is to distribute a 'base package' for each model organism with a sample of it. Each package includes all the basic vectors a person might need (characterized promoters, variety of markers). To put a gene in, you mix the CDS plasmid and a base package vector for an hour at 37c (with enzyme) and transform into a specific strain. That strain will be able to directly conjugate into whatever organism you are modifying.

I've finished the main base plasmid, just adding in the F1 origin for packaging this week, and hopefully be testing packaging by next week. It'll be a while until the full version is out since the A thaliana portion will take a while (characterizing method to directly conjugate with Agrobacterium and select on soil, avoiding Agrobacterium transformation and tissue culture) and I am also working out the exact efficiency details of using small synthetic DNA fragments in the goldengate reactions (to make the system a viable alternative to most other cloning methods)

-Koeng

On Saturday, May 14, 2016 at 8:32:42 AM UTC, Hiro Protagonist wrote:

Hi all,

I am interested in research with yeast, however it would be necessary to use a sequenced strain, which is available for private usage.

As it turned out one can actually get Saccharomyces cerevisiae stains (which would be my model organism of choice), however it appears that lab strains as S288c (which again would be the preferred strain) somehow seem to be classified as level 1 GMOs making them unavailable for non-institutational usage. 

Is there still any way to get access to these strains or does anyone know a fully sequenced strain of yeast with high homology to human proteins (especially mTOR), which doesn't require the customer to have access to level 1 biosafety labs?

Thanks in advance