Checked the recipe. Addition of Calcium carbonate allows for "easy" identification of acetogenic bacteria.
Il giorno mercoledì 15 giugno 2016 09:22:49 UTC+2, Pieter ha scritto:
-- Growth leads to excretion of acetic acid, with following medium acidification, releasing the carbonate from the calcium and forming "halos" around the bacteria.
No need for it if you have already isolated cultures.
Alcohol should be added as last, possibly filtered. A next step of boiling/cooking would just release it.
It works as fast food for G. xylinus, inducing cellulose production.
If you are interested in the biomaterial itself I'd strongly suggest to exchange glucose for glycerol.
As it is not fermentable yeast won't be able to produce gas and disrupt biofilm formation.
For isolation you could just use ethanol as carbon source.
Best,
Fede
Il giorno mercoledì 15 giugno 2016 09:22:49 UTC+2, Pieter ha scritto:
Here is medium recipe that we used: https://biohackacademy.github.io/bha3/annex/cultivation- media/acetobacter-medium/ In the SCOBY mother check for thick spots of cellulose. These often contain over producing strains. You can also select a bit on the plates.
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