Dang, hit post prematurely!
-- I posted the PFAM search result of TOR1 in my previous post. PFAM correctly identifies the kinase domain and reveals a number of other domains. You can take the PFAM accession number (e.g. PF11865) and look that up to see how TOR1 compares to molecules with related domains. Domains that are marked DUF (e.g. DUF3385 ) are domains of unknown functions.
What is cool is that you can often clone these domains out and study them independently. This can help you to divide and conquer your molecule. You can, for example, use a yeast two hybrid system with the different domains to see what binds to wildtype ATG13 vs the mutant forms.
Hope this helps,
Scott
http://www.opensciencenet.org
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