Re: [DIYbio] Re: A hypothetical protocol for DIYBIO DNA synthesis

My first thoughts were that the reversible terminator nucleotides
(whether heat or photo or acid labile/activated) are still too
expensive and single-source for this to be interesting for the effort,
at least in a DIYbio setting. I think the solution of the problem is
at the seam between chemistry and engineering... I am not sure if that
is simply materials science or chemical engineering at that point, but
it sounds pretty close.

On Tue, Jun 7, 2016 at 10:01 AM, CodeWarrior <code.w4rri0r@gmail.com> wrote:
> in my opinion the bottleneck in DNA synth is chemistry not engineering.
> Photo liable nucleotides mean you can dispense with the piezoelectric print
> head and use a projector system instead. but unless you can find somewhere
> to buy them or you can synthesise them easily yourself I'd say the chemical
> difficulty involved wouldn't justify the effort.
>
> On Tuesday, June 7, 2016 at 5:42:58 AM UTC+1, Felipe Tanaami wrote:
>>
>> Veeery interesting! I have a printer acumulating dust here, because it's
>> head is clogged. Now I have a good reason to try to clean it!
>> It's funny, I was researching exactly the same thing, I read that Cooper's
>> igem team paper last week and I was thinking on a way to make long sequences
>> with it. The microarray was a great idea! What do you think about the
>> photolabile protected nucleosides? It can simplify the work a lot, I
>> imagine. Keep us updated!
>
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--
-Nathan

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