I'm curious how you would exploit the nanopore to achieve synthesis?
On Friday, July 15, 2016 at 11:33:03 PM UTC+1, Nathan McCorkle wrote:
-- On Friday, July 15, 2016 at 11:33:03 PM UTC+1, Nathan McCorkle wrote:
On Fri, Jul 15, 2016 at 10:20 AM, CodeWarrior <code.w...@gmail.com> wrote:
> My apologies for perpetually spamming and bumping this thread. One more
> simplifying idea has occurred to me. Suppose the synthesised TdT enzyme had
> a long tail at one terminal which was then covalently bound to the glass
> slide along with the short oligo seeds pre DNA synthesis. No enzyme would
> then need to be deposed in each new cycle the existing TdT could be reused.
I've considered using nanopores, or a matrix of them, like a
strainer... they're cheap and easily orderable too for about $32 each
(they even sent me a free sample), from places like:
http://www.temwindows.com/product_p/sn100-p20q05.htm
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