Re: [DIYbio] Re: A hypothetical protocol for DIYBIO DNA synthesis

Ah that's workable for supper short sequences but the yield will be low and the needed input masive. Imagine you tune your TdT reaction perfectly and get a 30% yield of single nucliotide aditions (which I expect is optimistic). Then let's argue your seperation procedure is 100% lossless (highly unlikely). After 8 rounds you'll have less than 0.007% of the oligos you started with. You've also had to do 8 seperation a probably 8 consecutive PAGE procedures. It's just not efficient to add 8 nucliotides to a sequence. After that you need to jam a primer on the end and do some PCR or you'll be working with a sample diluted out of existence.

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