[DIYbio] Re: How to create a yeast extract from bread making yeast for medium?

Hard to say, but if you autoclave it, it should last for several weeks. As far as you keep it sterile should be good enough for your purposes. Keeping it in the fridge will help. Expect some debris on the bottom of your bottle if you store it, even if you filter it (I've seen it even in autoclaved 0.2um-filtered commercial yeast extract).

Good luck!

On Thursday, 1 December 2016 01:19:22 UTC+11, Aubrey wrote:
Do you know how long the extract will last with this?

On Wednesday, November 2, 2016 at 12:19:25 AM UTC-6, Scott wrote:
Hi Aubrey,

Interesting silk project. For your question have a look at this abstract.

When making media we normally autoclave (~120oC@~20minutes) the yeast extract (and the other components). Try boiling your media for awhile if you don't have a pressure cooker.

Let us know how your project goes.

Cheers,
Scott

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