Re: [DIYbio] Callus with Plant Tissue Culture

Thanks for the input!

 I would grow the callus on plain media.  MS basal salts, phytoagar, 3% sucrose, no hormones. 

So a callus will be able to self-differentiate into normal shoots without hormonal induction?

That *might* work but it will also mess with the seedling.  So I would grow the seedling and callus separately if you want the seedling to continue growling normally.

Out of curiosity, how will it mess with the seedling? I have some Drosera aliciae for example, seedlings that are much too small to separate from what appears to be developing callus cells.  

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