Re: [DIYbio] Homemade DIYBio: LB medium

I've used soy protein isolate to good effect. If you want, add bromelain (enzyme found in health stores) to "trypsinise" it.

On 3 February 2017 21:46:45 GMT+00:00, Willian Barela Costa <> wrote:
As everybody knows LB medium is the most common liquid media for culturing bacteria. For a biohacker is important to have in garage this medium for severeal experiences. 
Actually I have bioluminescent bacterias E.coli modified and I'm tring to think how produce LB medium at low cost.

Let's try hack - Formula: 

10g Trytone
5 g yeast extract
10g NaCl

Yeast extract and NaCl is cheap at the market, but the Trytone isn't. Thinking how produce trytone, basically is the use of a protease the trypsin in the casein. The casein is easy to obtein from milk, but the trypsin is the problem. Trypsin is usually found in digestive system of many vertebrates, for some sellers, they produce in E.Coli mofied and anothers extracted from herb (I don't know which herbs). Other form to approach this problem is to replace Trytone for other ingredient, I think to use products like Whey protein or use the own casein and pray to bacteria degrade.

Someone could help me to think in another solution or insight ?

ty for everybody pay attetion in this problem

Sent from my Android device with K-9 Mail. Please excuse my brevity.

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