Re: [DIYbio] Suggestions for beginner projects?

I personally like the last one on your list of ideas as a beginner
project (isolation of V. phosphoerum or V. fischeri from seafood), for
the following reasons:

* Lets you get started cheaply, with no need to order fancy stuff or
reagents
* Gives you some hands-on experience with bread&butter skills - pouring
agar plates, sterile technique etc
* Opportunities to dive deeper into questions about quorum sensing, or
culture media and conditions (e.g. by measuring the light output of a
colony over time with different culture media, temperature, oxygen
concentration, ...)

I also like the general approach of building skills on top of each other
- so, starting out with simple culturing of bacteria, and then moving on
to transformation. This gives you some confidence and competence to
figure out what's going on when you run into problems. You know, rather
than having to disentangle a ton of sources of error all at once with no
experience in any of them to guide you...

If you haven't come across it yet, also have a look at "I,
Microbiologist"
(https://www.amazon.de/Microbiologist-Discovery-Based-Undergraduate-Microbial-Molecular/dp/1555814700)

On 04.03.2017 18:56, MC wrote:
> Based on your experiences, what recommendations do you have for
> relatively simple beginner projects with good learning objectives that
> can be performed and verified without specialized equipment (like PCR)?
> Here are the ones I have in mind, any other ones you can suggest?
>
> * E. coli transformation with pVIB, GFP, pBLU, and other visually
> verifiable plasmids.
> * Agrobacterium mediated transformation of plants with GFP.
> * Isolation of V. phosphoreum from seafood.
>
> Thanks!
>
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