Re: [DIYbio] Suggestions for beginner projects?

I just took a look at the table of contents of that book and it looks like the perfect read for my level, thanks! And you've echoed my thoughts exactly, I've cultured a few strains and played with pVIB, but now I want to take it to the next step.

On Tuesday, March 7, 2017 at 12:27:49 AM UTC-8, lisa wrote:
I personally like the last one on your list of ideas as a beginner
project (isolation of V. phosphoerum or V. fischeri from seafood), for
the following reasons:

* Lets you get started cheaply, with no need to order fancy stuff or
* Gives you some hands-on experience with bread&butter skills - pouring
agar plates, sterile technique etc
* Opportunities to dive deeper into questions about quorum sensing, or
culture media and conditions (e.g. by measuring the light output of a
colony over time with different culture media, temperature, oxygen
concentration, ...)

I also like the general approach of building skills on top of each other
- so, starting out with simple culturing of bacteria, and then moving on
to transformation. This gives you some confidence and competence to
figure out what's going on when you run into problems. You know, rather
than having to disentangle a ton of sources of error all at once with no
experience in any of them to guide you...

If you haven't come across it yet, also have a look at "I,

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