Re: [DIYbio] DIY nanodrop alternative?

any news on this project?

On Tuesday, October 15, 2013 at 10:38:33 AM UTC-5, Sebastian wrote:
My next spec would be a project like that...using a set of standards like the Qubit (0 and 5ug) to calibrate the device and a really good ADC.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Sebastian Cocioba
Sent: 10/15/2013 11:36 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] DIY nanodrop alternative?

You could go the Qubit route and use a blue excitable intercalating dye like Midori Green and a blue LED. The expensive or hard to find part would be the sensor to detect the green within the blue. Filtering the light post excitation would help...something like an orange filter to block out the blue and let the green shine through. The sensor would need to be around the emission spectra which is very tight to the excitation freq, which is why most blue light boxes use an orange filter. The resolution would be low but comparable to older GeneQuant devices.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

From: Patrik D'haeseleer
Sent: 10/15/2013 3:00 AM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] DIY nanodrop alternative?

This still only for 600 nm, right? I keep thinking there should be a cheap hack to do a simple 260/280 nm spec for RNA/DNA quantitation and purity testing. Much more demand for that than for a general UV/Vis spectrophotometer. Maybe just a diffraction grating and two UV sensors at fixed angles?

Patrik

On Monday, October 14, 2013 6:39:12 AM UTC-7, Sebastian wrote:
I posted off list by accident. Here was my response to hugo's question:

So far so good. Still working on the integrated microcontroller PCB and
arduino shield alternative. I've been using my prototype for the last
few weeks making competent cells and such and it works quite well. The
only issue is that due to the arduino's crappy clock and edge detection
I lose some edges if you make a reading at the wrong time so I'm not
comfortable charging anything until I get this important issue fixed.
It does not hinder the performance, rather you may have to blank a few
times to get a 100% transmittance reading. I'd like to add a custom
circuit for edge detection so the only thing the arduino needs to do is
output to the LCD and down the USB as serial IO. Will start working on
that issue with more intensity now that I know someone is interested.
There are others on the list working on a similar device so they may
chime in too.


Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Cathal Garvey
Sent: 10/14/2013 9:28 AM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] DIY nanodrop alternative?
Don't go Kickstarter; they've already demonstrated a dislike of
Science. Go with any of the other crowdfunders or selfstarter.

I will be happy to snap one of these up; I still have no working spec
and could use one.

On Mon, 14 Oct 2013 05:37:29 -0700 (PDT)
Hugo Correia <har...@gmail.com> wrote:

> So what's the status on this? Kickstart ready?
>
> On Sunday, 14 July 2013 07:45:36 UTC+2, Sebastian wrote:
> >
> >  I'm in the process of making a very affordable dedicated OD600
> > module soon. I can send you a prototype to demo free of charge. Its
> > all open source. The final kit will be 25 bucks and initial trials
> > are proving quite good. Its fixed at 600nm but will have lots of
> > nice features and the ability to swap modules for other wavelengths
> > or multiple cuvettes. If all goes well I may kick start it to make
> > one for DNA. Ill keep you posted if you'd wish.
> >
> > Sent from my Windows Phone
> >  ------------------------------
> > From: Koeng <javascript:>
> > Sent: 7/14/2013 12:52 AM
> > To: diy...@googlegroups.com <javascript:>
> > Subject: [DIYbio] DIY nanodrop alternative?
> >
> > Hey!
> >
> > I have been looking around for a spectrometer for my lab, and
> > wanted to know if anyone has any recommendations on one. All I need
> > is it to have OD600 and most of all, I want it to be able to sense
> > how much DNA I have in a sample. I am not very experienced with
> > spectrometers, so any suggestions?
> >
> > --
> > -- You received this message because you are subscribed to the
> > Google Groups DIYbio group. To post to this group, send email to
> > diy...@googlegroups.com <javascript:>. To unsubscribe from this
> > group, send email to diybio+un...@googlegroups.com <javascript:>.
> > For more options, visit this group at
> > https://groups.google.com/d/forum/diybio?hl=en
> > Learn more at www.diybio.org
> > ---
> > You received this message because you are subscribed to the Google
> > Groups "DIYbio" group.
> > To unsubscribe from this group and stop receiving emails from it,
> > send an email to diybio+un...@googlegroups.com <javascript:>.
> > To post to this group, send email to diy...@googlegroups.com
> > <javascript:> .
> > Visit this group at http://groups.google.com/group/diybio.
> > To view this discussion on the web visit
> > https://groups.google.com/d/msgid/diybio/0707dd13-ed86-4f83-8984-0a7e140111f0%40googlegroups.com
> > .
> > For more options, visit https://groups.google.com/groups/opt_out.
> >
> >
> >
>

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/926a595a-a561-4f70-9a64-6a73b2474bd6%40googlegroups.com.
For more options, visit https://groups.google.com/groups/opt_out.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/1fd74d56-3d67-4c32-9514-ca3c54ff8d40%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment