Re: [DIYbio] Private Cheap Genome Sequencing

Getting the genome sequenced is most likely going to be the easy portion of this process. If you want to process all that data that is going to come back, which is generally in the form of 150-450 base pair segments that are then overlapped based on sequence continuity, you are either going to need to contact a company that will both sequence and process the data for you (which will be more expensive), or find a way to search for what you are looking for yourself. 

The hard part about this, is that the raw sequences will most likely be sent to you in a bunch of packets of roughly 50,000 sequences that you'll have to search through to find what you want. To process a single one of these would be simple, but to process all of them at once would require a computer program that utilizes a distributed framework (i.e. Hadoop), unless you happen to have a super computer or server system just laying around. 

To summarize - getting lots of sequence is not difficult, but finding something in it (your needle in the needle stack) is more difficult and requires some programming knowledge (it's a bit more complex than Crtl+F).

I hope this helps. 

-Skyler 

On Sat, Jul 8, 2017 at 7:38 AM, Abizar Lakdawalla <abizarl@gmail.com> wrote:

send it out to get sequenced, a whole genome costs about us$1500 to 3000. a whole exome (protein coding part of the genome) costs about $500 ish. you get a list of variants plus the raw data.

On Jul 8, 2017 3:02 AM, "Mary Ward" <maryhbw@gmail.com> wrote:

Hi Folks,

I want to sequence my genome, but I want to keep the information confidential. I am interested in learning about some gene markers associated with health and genealogy.

I would like to do this without making the bizzilion primers to do this on a hacker scale, but I am not opposed if there is a primer library for me to reference.

Recommendations?

THANK YOU :)

-Mary, maryhbw@gmail.com

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