Re: [DIYbio] Camera for fluorescence detection system

On 11/23/20 4:23 PM, 'Charles Maldonado' via DIYbio wrote:
> The moving particles are in a tube with fixed wall thickness. These particles are only moving in one direction and have a constant
> speed. So it's fine that I only measure the fluorescence directly in front of my sensor. I have to detect their presence/absence.
> I am triggering the fluorescence by illuminating the particles with light from a LED. I want to place my sensor perpendicular to
> the light source.

Since you say you don't need to know how strongly they glow, or if there are two of them clumped together, you could get away
without image processing.

" I only have to detect one fluorophore. "

Will there be a low low chance of two touching or close to each other?

If so, the sensor route is good. You might even be able to detect when two are in the field of view by relative intensities. Is
the light from one particle varying a lot if it is against the far wall of the tube or near wall? Are you wanting to do any
hardware or code development, or just "full manual" proof of concept?

John

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