[DIYbio] Line item success fail rate

Anytime the question is asked ? "How often does this succeed ?" The usual answer starts with a long observation; ex: "It depends ...". Ok

Recall from a strict numerical method approach, if success or failure criterea can be decided upon per act, and acts batched into some kind, there is always a number in the noise, and an important one. What is the line item success rate, as usually composed ? 

 Sure some if;s in the question ! Situation:

*) Gene BP count is 3K NT, promotor tails, introns if any etc well known to work.
*) Expression system known, like locally managed Yeast or 'whatever'
*) Selection itself is reliable, UV florescence or some other property

 Q1) How many labor hours is typical, no paperwork time, just wet lab including end game verification ?
 Q2) Whats the success rate of a new germ line in percent ?
 Q3) How often is a success rate wrong ultimately. not that the Gene did do what is expected, but some 'other' flaw made it not really get expressed ?
 Q4) Is sequencing the believed usable final modified colony invariably reliable enough to make Q3 irrelevant ?

Thanks in advance of anyone who answers. As people like me want to make this technology, not science, this is a big thing hugely.

is Q2) Closer to what ? 30% 80 %

For Q1) I mean hands on time with endless revisits to the plates and so on, not includng incubation times, centerfuge, etc.

Thanks! 

Daniel B Kolis

my ref: nafl, 30 Aug 2022, diybio